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Myeloperoxidase (MPO) Antibody and Serine Proteinase 3 (PR3) Antibody with Reflex to Anti-Neutrophil Cytoplasmic Antibody, IgG by IFA

ANCA-PN
Example Reports
Negative
Positive

Ordering Recommendation

Preferred first-line reflex panel for the evaluation of ANCA-associated vasculitis.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

CSF, plasma, urine, or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Methodology

Semi-Quantitative Indirect Fluorescent Antibody (IFA)/Semi-Quantitative Multiplex Bead Assay

Performed

Sun-Sat

Reported

2-5 days

Reference Interval

Test Number
 Components
 Reference Interval
  Myeloperoxidase (MPO) Ab, IgG 19 AU/mL or less
  Serine Proteinase 3 (PR3) Ab, IgG 19 AU/mL or less

Interpretive Data




Component Interpretation
Myeloperoxidase (MPO) Antibody 19 AU/mL or less  Negative
20-25 AU/mL  Equivocal
26 AU/mL or greater   Positive
Serine Proteinase 3 (PR3) Antibody 19 AU/mL or less  Negative
20-25 AU/mL  Equivocal
26 AU/mL or greater   Positive

Compliance Category

FDA

Note

If MPO and/or PR3 results are equivocal or positive, then ANCA by IFA will be added. Additional changes apply. Specimens are screened by IFA on ethanol-fixed neutrophils, formalin-fixed neutrophils, and HEp-2 slides that allow differentiation of C- and P-ANCA patterns.

Hotline History

N/A

CPT Codes

83516 x2; if reflexed, add 86036

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Components

Component Test Code* Component Chart Name LOINC
0050526 Myeloperoxidase (MPO) Ab, IgG 6969-0
0050527 Serine Proteinase 3 (PR3) Ab, IgG 6968-2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • (Perinuclear Antineutrophil Cytoplasmic Antibody
  • ACPA
  • ANCA
  • ANCA Panel, IgG
  • Anti-Neutrophil Cytoplasmic Ab Test
  • Anti-Neutrophil Cytoplasmic Antibodies Panel, IgG
  • Anticytoplasmic Antibody
  • Antineutrophil Cytoplasmic Antibodies (ACPA)
  • Autoantibodies to Proteinase 3
  • c ANCA (Anti-Neutrophil Cytoplasmic Antibody with Reflex to Titer & MPO/PR3 Antibodies)
  • c-ANCA
  • Cytoplasmic Neutrophil Antibodies; MPO)
  • p ANCA
  • P-ANCA
  • pANCA
  • PR3
  • Proteinase 3
Myeloperoxidase (MPO) Antibody and Serine Proteinase 3 (PR3) Antibody with Reflex to Anti-Neutrophil Cytoplasmic Antibody, IgG by IFA